Chemopreventive activities of sulforaphane and its metabolites in human hepatoma HepG2 cells

Liu, Peng, Wang, Wei, Zhou, Zhigang, Smith, Andrew, Bowater, Richard P. ORCID: https://orcid.org/0000-0002-2745-7807, Wormstone, Ian Michael ORCID: https://orcid.org/0000-0002-6423-7766, Chen, Yuqiong and Bao, Yongping ORCID: https://orcid.org/0000-0002-6425-0370 (2018) Chemopreventive activities of sulforaphane and its metabolites in human hepatoma HepG2 cells. Nutrients, 10 (5). ISSN 2072-6643

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Abstract

Sulforaphane (SFN) exhibits chemopreventive effects through various mechanisms. However, few studies have focused on the bioactivities of its metabolites. Here, three metabolites derived from SFN were studied, known as sulforaphane glutathione, sulforaphane cysteine and sulforaphane-N-acetylcysteine. Their effects on cell viability, DNA damage, tumorigenicity, cell migration and adhesion were measured in human hepatoma HepG2 cells, and their anti-angiogenetic effects were determined in a 3D co-culture model of human umbilical vein endothelial cells (HUVECs) and pericytes. Results indicated that these metabolites at high doses decreased cancer cell viability, induced DNA damage and inhibited motility, and impaired endothelial cell migration and tube formation. Additionally, pre-treatment with low doses of SFN metabolites protected against H₂O₂ challenge. The activation of the nuclear factor E2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway and the induction of intracellular glutathione (GSH) played an important role in the cytoprotective effects of SFN metabolites. In conclusion, SFN metabolites exhibited similar cytotoxic and cytoprotective effects to SFN, which proves the necessity to study the mechanisms of action of not only SFN but also of its metabolites. Based on the different tissue distribution profiles of these metabolites, the most relevant chemical forms can be selected for targeted chemoprevention.

Item Type:	Article
Uncontrolled Keywords:	sulforaphane,chemoprevention,sulforaphane metabolites,nrf2 or nuclear factor erythroid 2 [nf-e2]-related factor 2,gsh,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Biological Sciences Faculty of Science > School of Natural Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Biosciences Teaching and Education Research Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Cells and Tissues Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	Pure Connector
Date Deposited:	25 May 2018 14:30
Last Modified:	13 Nov 2023 17:43
URI:	https://ueaeprints.uea.ac.uk/id/eprint/67188
DOI:	10.3390/nu10050585

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