Minimal sampling protocol for accurate estimation of urea production: a study with oral [13C]urea in fed and fasted piglets

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Oosterveld, Michiel J.S., Gemke, Reinoud J.B.J., Dainty, Jack R. ORCID: https://orcid.org/0000-0002-0056-1233, Kulik, Willem, Jakobs, Cornelis and de Meer, Kees (2005) Minimal sampling protocol for accurate estimation of urea production: a study with oral [13C]urea in fed and fasted piglets. Clinical Nutrition, 24 (1). pp. 97-104. ISSN 0261-5614

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Abstract

Background & aims: An oral [13C]urea protocol may provide a simple method for measurement of urea production. The validity of single pool calculations in relation to a reduced sampling protocol was assessed. Methods: In eight fed and five fasted piglets, plasma urea enrichments from a 10 h sampling protocol were measured following an intragastric [13C]urea bolus. Blood [13C]bicarbonate was measured to trace gut [13C]urea oxidation. Two-compartment and regression (single pool) computations were performed. Pool sizes were compared to urea distribution over total body water (TBW). Shorter protocol duration was tested in regression simulations. Results: Differences in urea kinetics between fed and fasted piglets did not reach statistical significance. Mean (±SE) urea pool from TBW times plasma urea concentration was 2.2±0.16 mmol kg−1. Two-compartment modelling yielded similar results for pool size (despite the oxidation of a small amount of urea tracer). Urea appearance rate was 306±18 μmol kg−1 h−1. Regression calculations overestimated urea appearance rate vs. compartmental model (). When samples <2 h were discarded, results were comparable to compartmental calculations even if protocol length was 6 h (325±24 μmol kg−1 h−1, NS). Conclusions: Regression calculations using plasma enrichments sampled between 2 and 6 h after oral [13C]urea administration provide accurate rates of urea production, and are not affected by tracer oxidation.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: Pure Connector
Date Deposited: 27 Feb 2018 15:30
Last Modified: 22 Oct 2022 03:36
URI: https://ueaeprints.uea.ac.uk/id/eprint/66390
DOI: 10.1016/j.clnu.2004.07.017

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