Tools
Tools
Tian, Sicong, Li, Baolong, Lei, Peng, Yang, Xiuli, Zhang, Xiaohong, Bao, Yongping 
ORCID: https://orcid.org/0000-0002-6425-0370 and Shan, Yujuan
(2018)
Sulforaphane improves abnormal lipid metabolism via both ERS-dependent XBP1/ACC &SCD1 and ERS-independent SREBP/FAS pathways.
Molecular Nutrition & Food Research, 62 (6).
ISSN 1613-4125
Preview |
PDF (Accepted manuscript)
- Accepted Version
Available under License Creative Commons Attribution Non-commercial. Download (1MB) | Preview |
Abstract
Scope: To investigate the effect of sulforaphane (SFN) on the abnormal lipid metabolism and underlying mechanisms. Methods and results: Models with abnormal lipid metabolism were established both in rats and human hepatocytes. Hepatic steatosis was detected by H&E and oil red O staining. The structure of endoplasmic reticulum was visualized by transmission electron microscopy. The expressions of X-box binding protein 1 (XBP1), protein kinase-like ER kinase (PERK), sterol regulatory element binding protein-1c (SREBP1c) and lipogenic enzymes were determined by real-time PCR and western blot analysis. SFN lowered the content of triglyceride and cholesterol. SFN alleviated the swelling of endoplasmic reticulum (ER) and decreased the perimeter of ER. SFN significantly decreased the expressions of acetyl CoA carboxylase 1 (ACC1), stearoyl-CoA desaturase 1 (SCD1) and fatty acid synthase. SFN inhibited SREBP1c by blocking the PERK. Meanwhile, SFN suppressed ACC1 and SCD1 via blocking the formation of splicing-type XBP1. The key roles of XBP1 and SREBP1c in SFN-reduced lipid droplets were confirmed by a timed sequence of measurement according to time points. Conclusion: SFN improved abnormal lipid metabolism via both ER stress -dependent and -independent pathways.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | endoplasmic reticulum stress,lipid metabolism,lipogenic enzymes,sterol regulatory element binding protein-1c,sulforaphane |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 01 Feb 2018 10:30 |
| Last Modified: | 13 Nov 2023 17:42 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/66170 |
| DOI: | 10.1002/mnfr.201700737 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |