Dominant integration locus drives continuous diversification of plant immune receptors with exogenous domain fusions

Bailey, Paul C., Schudoma, Christian, Jackson, William, Baggs, Erin, Dagdas, Gulay, Haerty, Wilfried ORCID: https://orcid.org/0000-0003-0111-191X, Moscou, Matthew ORCID: https://orcid.org/0000-0003-2098-6818 and Krasileva, Ksenia V. (2018) Dominant integration locus drives continuous diversification of plant immune receptors with exogenous domain fusions. Genome Biology, 19. ISSN 1474-760X

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Abstract

Background: The plant immune system is innate and encoded in the germline. Using it efficiently, plants are capable of recognizing a diverse range of rapidly evolving pathogens. A recently described phenomenon shows that plant immune receptors are able to recognize pathogen effectors through the acquisition of exogenous protein domains from other plant genes. Results: We show that plant immune receptors with integrated domains are distributed unevenly across their phylogeny in grasses. Using phylogenetic analysis, we uncover a major integration clade, whose members underwent repeated independent integration events producing diverse fusions. This clade is ancestral in grasses with members often found on syntenic chromosomes. Analyses of these fusion events reveals that homologous receptors can be fused to diverse domains. Furthermore, we discover a 43 amino acids long motif associated with this dominant integration clade and that is located immediately upstream of the fusion site. Sequence analysis reveals that DNA transposition and/or ectopic recombination are the most likely mechanisms of formation for Nucleotide Binding Leucine Rich Repeat proteins with Integrated Domains. Conclusions: The identification of this subclass of plant immune receptors that is naturally adapted to new domain integration will inform biotechnological approaches for generating synthetic receptors with novel pathogen 'baits'.

Item Type:	Article
Uncontrolled Keywords:	plant immunity,disease resistance genes,nlrs,gene fusions
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > The Sainsbury Laboratory
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging
Depositing User:	Pure Connector
Date Deposited:	18 Jan 2018 10:30
Last Modified:	23 Oct 2024 23:48
URI:	https://ueaeprints.uea.ac.uk/id/eprint/65984
DOI:	10.1186/s13059-018-1392-6

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