Lifelong calorie restriction affects indicators of colonic health in aging C57Bl/6J mice

Kok, Dieuwertje E. G., Rusli, Fenni, van der Lugt, Benthe, Lute, Carolien, Laghi, Luca, Salvioli, Stefano, Picone, Gianfranco, Franceschi, Claudio, Smidt, Hauke, Vervoort, Jacques, Kampman, Ellen, Müller, Michael ORCID: https://orcid.org/0000-0002-5930-9905 and Steegenga, Wilma T. (2018) Lifelong calorie restriction affects indicators of colonic health in aging C57Bl/6J mice. Journal of Nutritional Biochemistry, 56. 152–164. ISSN 0955-2863

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Abstract

Diminished colonic health is associated with various age-related pathologies. Calorie restriction (CR) is an effective strategy to increase healthy lifespan, although underlying mechanisms are not fully elucidated. Here, we report the effects of lifelong CR on indicators of colonic health in aging C57Bl/6J mice. Compared to an ad libitum control and moderate-fat diet, 30% energy reduction was associated with attenuated immune- and inflammation-related gene expression in the colon. Furthermore, expression of genes involved in lipid metabolism was higher upon CR, which may point towards efficient regulation of energy metabolism. The relative abundance of bacteria considered beneficial to colonic health, such as Bifidobacterium and Lactobacillus, increased in the mice exposed to CR for 28 months as compared to the other diet groups. We found lower plasma levels of interleukin 6 and lower levels of various metabolites, among which bile acids, in the colonic luminal content of CR-exposed mice as compared to the other diet groups. Switching from CR to an ad libitum moderate-fat diet at old age (24 months) revealed remarkable phenotypic plasticity in terms of gene expression, microbiota composition and metabolite levels, although expression of a subset of genes remained CR-associated. This study demonstrated in a comprehensive way that CR affects indicators of colonic health in aging mice. Our findings provide unique leads for further studies that need to address optimal and feasible strategies for prolonged energy deprivation, which may contribute to healthy aging.

Item Type:	Article
Uncontrolled Keywords:	aging,calorie restriction,colonic health,gene expression,gut microbiota,metabolites
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	Pure Connector
Date Deposited:	16 Jan 2018 14:30
Last Modified:	06 Jun 2024 15:00
URI:	https://ueaeprints.uea.ac.uk/id/eprint/65946
DOI:	10.1016/j.jnutbio.2018.01.001

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