Tools
Tools
Sivapalan, Tharsini, Melchini, Antonietta, Saha, Shikha, Needs, Paul W., Traka, Maria H., Tapp, Henri S., Dainty, Jack R. 
ORCID: https://orcid.org/0000-0002-0056-1233 and Mithen, Richard F.
(2018)
Bioavailability of glucoraphanin and sulforaphane from high-glucoraphanin broccoli.
Molecular Nutrition & Food Research, 62 (18).
ISSN 1613-4125
Preview |
PDF (Published manuscript)
- Published Version
Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
Scope: Broccoli accumulates 4-methylsulphinylbutyl glucosinolate (glucoraphanin) which is hydrolyzed to the isothiocyanate sulforaphane. Through the introgression of novel alleles of the Myb28 transcription factor from Brassica villosa, broccoli genotypes have been developed that have enhanced levels of glucoraphanin. This study sought to quantify the exposure of human tissues to glucoraphanin and sulforaphane following consumption of broccoli with contrasting Myb28 genotypes. Methods and results: Ten participants were recruited into a three-phase, double-blinded, randomized crossover trial (NCT02300324), with each phase comprising consumption of 300 ml of a soup made from broccoli of one of three Myb28 genotypes (Myb28B/B, Myb28B/V, Myb28V/V). Plant myrosinases were intentionally denatured during soup manufacture. Three-fold and five-fold higher levels of sulforaphane occurred in the circulation following consumption of Myb28V/B and Myb28V/V broccoli soups, respectively. The percentage of sulforaphane excreted in 24 h relative to the amount of glucoraphanin consumed varied amongst volunteers from 2% to 15%, but did not depend on the broccoli genotype. Conclusion: This is the first study to report the bioavailability of glucoraphanin and sulforaphane from soups made with novel broccoli varieties. The presence of one or two Myb28V alleles results in enhanced delivery of sulforaphane to the systemic circulation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | bioavailability,broccoli,glucoraphanin,pharmacokinetics,sulforaphane |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Pharmacy |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Population Health |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 15 Jan 2018 16:30 |
| Last Modified: | 06 Jun 2024 15:00 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/65934 |
| DOI: | 10.1002/mnfr.201700911 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |