The molecular bases of the dual regulation of bacterial iron sulfur cluster biogenesis by CyaY and IscX

Adinolfi, Salvatore, Puglisi, Rita, Crack, Jason, Iannuzzi, Clara, Dal Piaz, Fabrizio, Konarev, Petr V., Svergun, Dmitri I., Martin, Stephen, Le Brun, Nick E. ORCID: https://orcid.org/0000-0001-9780-4061 and Pastore, Annalise (2018) The molecular bases of the dual regulation of bacterial iron sulfur cluster biogenesis by CyaY and IscX. Frontiers in Molecular Biosciences, 4. ISSN 2296-889X

Preview

PDF (Published manuscript) - Published Version Available under License Creative Commons Attribution. Download (5MB) | Preview

Abstract

IscX (or YfhJ) is a protein of unknown function which takes part in the iron-sulfur cluster assembly machinery, a highly specialised and essential metabolic pathway. IscX binds to iron with low affinity and interacts with IscS, the desulfurase central to cluster assembly. Previous studies have suggested a competition between IscX and CyaY, the bacterial ortholog of frataxin, for the same binding surface of IscS. This competition could suggest a link between the two proteins with a functional significance. Using a hybrid approach based on nuclear magnetic resonance, small angle scattering and biochemical methods, we show here that IscX is a modulator of the inhibitory properties of CyaY: by competing for the same site on IscS, the presence of IscX rescues the rates of enzymatic cluster formation which are inhibited by CyaY. The effect is stronger at low iron concentrations, whereas it becomes negligible at high iron concentrations. These results strongly suggest the mechanism of the double regulation of iron sulfur cluster assembly under the control of iron as the effector.

Item Type:	Article
Uncontrolled Keywords:	desulfurase,enzyme activity,frataxin,isc operon,iron chaperone,iron sulfur cluster
Faculty \ School:	Faculty of Science > School of Chemistry (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Chemistry of Life Processes
Related URLs:	https://www.frontiersin.org/articles/10....
Depositing User:	Pure Connector
Date Deposited:	11 Jan 2018 11:30
Last Modified:	20 Dec 2024 00:57
URI:	https://ueaeprints.uea.ac.uk/id/eprint/65911
DOI:	10.3389/fmolb.2017.00097

Downloads

Actions (login required)

View Item