Frizzled-7 is required for Xenopus heart development

Abu-Elmagd, Muhammad, Mulvaney, Joanna and Wheeler, Grant N (2017) Frizzled-7 is required for Xenopus heart development. Biology Open, 6 (12). pp. 1861-1868. ISSN 2046-6390

[img]
Preview
PDF (Accepted manuscript) - Submitted Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview
[img]
Preview
PDF (BiologyOpen_2017_6_1861) - Published Version
Available under License Creative Commons Attribution.

Download (2MB) | Preview

Abstract

Wnt signalling regulates cardiogenesis during specification of heart tissue and the morphogenetic movements necessary to form the linear heart. Wnt11 mediated non-canonical signalling promotes early cardiac development whilst Wnt11-R, which is expressed later, also signals through the non-canonical pathway to promote heart development. It is unclear which Frizzleds mediate these interactions. Frizzled-7 (fzd7) is expressed during gastrulation in the mesodermal cells fated to become heart and then in the primary heart field. This expression is complementary to the expression of wnt11 and wnt11-R We further show co-localisation of fzd7 with other early and late heart-specific markers using double in situ hybridisation. We have used loss of function analysis to determine the role of fzd7 during heart development. Morpholino antisense oligonucleotide-mediated knockdown of Fzd7 results in effects on heart development, similar to that caused by Wnt11 loss of function. Surprisingly, overexpression of dominant-negative Fzd7 cysteine rich domain (Fzd7 CRD) results in a cardia bifida phenotype, similar to the loss of wnt11-R phenotype. Overexpression of Fzd7 and activation of non-canonical wnt signalling can rescue the effect of Fzd7 CRD. We propose that Fzd7 has an important role during Xenopus heart development.

Item Type: Article
Additional Information: © 2017. Published by The Company of Biologists Ltd.
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 05 Dec 2017 06:07
Last Modified: 19 Aug 2020 23:58
URI: https://ueaeprints.uea.ac.uk/id/eprint/65671
DOI: 10.1242/bio.026963

Actions (login required)

View Item View Item