Frizzled-7 is required for Xenopus heart development

Abu-Elmagd, Muhammad, Mulvaney, Joanna and Wheeler, Grant N. ORCID: https://orcid.org/0000-0002-4335-8577 (2017) Frizzled-7 is required for Xenopus heart development. Biology Open, 6 (12). pp. 1861-1868. ISSN 2046-6390

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Abstract

Wnt signalling regulates cardiogenesis during specification of heart tissue and the morphogenetic movements necessary to form the linear heart. Wnt11 mediated non-canonical signalling promotes early cardiac development whilst Wnt11-R, which is expressed later, also signals through the non-canonical pathway to promote heart development. It is unclear which Frizzleds mediate these interactions. Frizzled-7 (fzd7) is expressed during gastrulation in the mesodermal cells fated to become heart and then in the primary heart field. This expression is complementary to the expression of wnt11 and wnt11-R We further show co-localisation of fzd7 with other early and late heart-specific markers using double in situ hybridisation. We have used loss of function analysis to determine the role of fzd7 during heart development. Morpholino antisense oligonucleotide-mediated knockdown of Fzd7 results in effects on heart development, similar to that caused by Wnt11 loss of function. Surprisingly, overexpression of dominant-negative Fzd7 cysteine rich domain (Fzd7 CRD) results in a cardia bifida phenotype, similar to the loss of wnt11-R phenotype. Overexpression of Fzd7 and activation of non-canonical wnt signalling can rescue the effect of Fzd7 CRD. We propose that Fzd7 has an important role during Xenopus heart development.

Item Type:	Article
Additional Information:	© 2017. Published by The Company of Biologists Ltd.
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Cells and Tissues Faculty of Science > Research Groups > Wheeler Group
Depositing User:	Pure Connector
Date Deposited:	05 Dec 2017 06:07
Last Modified:	25 Sep 2024 13:09
URI:	https://ueaeprints.uea.ac.uk/id/eprint/65671
DOI:	10.1242/bio.026963

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