Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus

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Qi, Xiaoxuan, Wang, Wenjian, Dong, Haohao, Liang, Yuying, Dong, Changjiang and Ly, Hinh (2017) Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus. In: Hemorrhagic Fever Viruses. Hemorrhagic Fever Viruses . Humana Press, pp. 179-188. ISBN 978-1-4939-6980-7

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Abstract

We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.

Item Type: Book Section
Uncontrolled Keywords: sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
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Depositing User: Pure Connector
Date Deposited: 19 Oct 2017 05:08
Last Modified: 21 Mar 2024 02:33
URI: https://ueaeprints.uea.ac.uk/id/eprint/65185
DOI: 10.1007/978-1-4939-6981-4_12

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