Differential epitope mapping by STD NMR spectroscopy to reveal the nature of protein–ligand contacts

Monaco, Serena, Tailford, Louise E., Juge, Nathalie and Angulo, Jesus ORCID: https://orcid.org/0000-0001-7250-5639 (2017) Differential epitope mapping by STD NMR spectroscopy to reveal the nature of protein–ligand contacts. Angewandte Chemie-International Edition, 129 (48). 15491–15495. ISSN 1433-7851

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Abstract

Saturation transfer difference (STD) NMR spectroscopy is extensively used to obtain epitope maps of ligands binding to protein receptors, thereby revealing structural details of the interaction, which is key to direct lead optimization efforts in drug discovery. However, it does not give information about the nature of the amino acids surrounding the ligand in the binding pocket. Herein, we report the development of the novel method differential epitope mapping by STD NMR (DEEP-STD NMR) for identifying the type of protein residues contacting the ligand. The method produces differential epitope maps through 1) differential frequency STD NMR and/or 2) differential solvent (D2O/H2O) STD NMR experiments. The two approaches provide different complementary information on the binding pocket. We demonstrate that DEEP-STD NMR can be used to readily obtain pharmacophore information on the protein. Furthermore, if the 3D structure of the protein is known, this information also helps in orienting the ligand in the binding pocket.

Item Type:	Article
Uncontrolled Keywords:	protein-ligand binding,saturation transfer difference nmr,fragment based drug design,ligand pharmacophore,nmr spectroscopy
Faculty \ School:	Faculty of Science > School of Pharmacy
UEA Research Groups:	Faculty of Science > Research Groups > Drug Delivery and Pharmaceutical Materials (former - to 2017) Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter
Depositing User:	Pure Connector
Date Deposited:	06 Oct 2017 07:13
Last Modified:	29 Mar 2024 02:12
URI:	https://ueaeprints.uea.ac.uk/id/eprint/65094
DOI:	10.1002/ange.201707682

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