Enhanced bacterial wilt resistance in potato through expression of Arabidopsis EFR and introgression of quantitative resistance from Solanum commersonii

Boschi, Federico, Schvartzman, Claudia, Murchio, Sara, Ferreira, Virginia, Siri, Maria I., Galván, Guillermo A., Smoker, Matthew, Stansfeld, Lena, Zipfel, Cyril, Vilaró, Francisco L. and Dalla-Rizza, Marco (2017) Enhanced bacterial wilt resistance in potato through expression of Arabidopsis EFR and introgression of quantitative resistance from Solanum commersonii. Frontiers in Plant Science, 8. ISSN 1664-462X

Preview

PDF (Published manuscript) - Published Version Available under License Creative Commons Attribution. Download (3MB) | Preview

Abstract

Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato.

Item Type:	Article
Uncontrolled Keywords:	ralstonia solanacearum,bacterial wilt,solanum tuberosum,solanum commersonii,pattern recognition receptor,efr,quantitative resistance
Faculty \ School:	Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Plant Sciences
Depositing User:	Pure Connector
Date Deposited:	13 Sep 2017 05:06
Last Modified:	04 May 2024 12:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/64822
DOI:	10.3389/fpls.2017.01642

Downloads

Actions (login required)

View Item