A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana

Kirkham, Amy, Richthammer, Patrick, Schmidt, Katrin, Wustman, Martin, Maeda, Yoshiaki, Hedrich, Rene, Brunner, Eike, Tanaka, Tsuyoshi, van Pee, Karl-Heinz, Falciatore, Angela and Mock, Thomas (2017) A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana. The ISME Journal, 11 (11). pp. 2452-2464. ISSN 1751-7362

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Abstract

Diatoms contribute 20% of global primary production and form the basis of many marine food webs. Although their species diversity correlates with broad diversity in cell size, there is also an intraspecific cell-size plasticity due to sexual reproduction and varying environmental conditions. However, despite the ecological significance of the diatom cell size for food-web structure and global biogeochemical cycles, our knowledge about genes underpinning the size of diatom cells remains elusive. Here, a combination of reverse genetics, experimental evolution and comparative RNA8 sequencing analyses enabled us to identify a previously unknown genetic control of cell size in the diatom Thalassiosira pseudonana. In particular, the targeted deregulation of the expression of the cell-wall protein silacidin caused a significant increase in valve diameter. Remarkably, the natural downregulation of the silacidin gene transcript due to experimental evolution under low temperature also correlated with cell-size increase. Our data give first evidence for a genetically controlled regulation of cell size in Thalassiosira pseudonana and possibly other centric diatoms as they also encode the silacidin gene in their genomes.

Item Type: Article
Faculty \ School: Faculty of Science > School of Environmental Sciences
Depositing User: Pure Connector
Date Deposited: 21 Jul 2017 05:03
Last Modified: 31 Oct 2019 15:12
URI: https://ueaeprints.uea.ac.uk/id/eprint/64178
DOI: 10.1038/ismej.2017.100

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