Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

Nekrasov, Vladimir, Wang, Congmao, Win, Joe, Lanz, Christa, Weigel, Detlef and Kamoun, Sophien (2017) Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion. Scientific Reports, 7. ISSN 2045-2322

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Abstract

Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/Cas9 being a highly precise tool, as we did not detect off-target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.

Item Type: Article
Faculty \ School:
Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: Pure Connector
Date Deposited: 20 Jul 2017 05:05
Last Modified: 13 Oct 2019 00:24
URI: https://ueaeprints.uea.ac.uk/id/eprint/64169
DOI: 10.1038/s41598-017-00578-x

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