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Nekrasov, Vladimir, Wang, Congmao, Win, Joe, Lanz, Christa, Weigel, Detlef and Kamoun, Sophien 
ORCID: https://orcid.org/0000-0002-0290-0315
(2017)
Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion.
Scientific Reports, 7.
ISSN 2045-2322
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Abstract
Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/Cas9 being a highly precise tool, as we did not detect off-target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 20 Jul 2017 05:05 |
| Last Modified: | 21 Oct 2022 13:31 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/64169 |
| DOI: | 10.1038/s41598-017-00578-x |
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