Cell interactions, signals and transcriptional hierarchy governing placode progenitor induction

Hintze, Mark, Singh Prajapati, Ravindra, Tambalo, Monica, Christophorou, Nicolas, Anwar, Maryam, Grocott, Timothy ORCID: https://orcid.org/0000-0002-6321-401X and Streit, Andrea (2017) Cell interactions, signals and transcriptional hierarchy governing placode progenitor induction. Development, 144 (15). pp. 2810-2823. ISSN 0950-1991

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Abstract

In vertebrates, cranial placodes contribute to all sense organs and sensory ganglia and arsise from a common pool of Six1/Eya2+ progenitors. Here we dissect the events that specify ectodermal cells as placode progenitors using newly identified genes upstream of the Six/Eya complex. We show that two different tissues, the lateral head mesoderm and the prechordal mesendoderm, gradually induce placode progenitors: cells pass through successive transcriptional states, each identified by distinct factors and controlled by different signals. Both tissues initiate a common transcriptional state, but over time impart regional character and the acquisition of anterior identity depends on Shh signalling. Using a network inference approach we predict the regulatory relationships among newly identified transcription factors and verify predicted links using knock-down experiments. Based on this analysis we propose a new model for placode progenitor induction, in which the initial induction of a generic transcriptional state precedes regional divergence.

Item Type: Article
Uncontrolled Keywords: chick embryo,cell fate,sense organs,sensory ganglia,transcriptional networks, signalling
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 14 Jul 2017 05:06
Last Modified: 22 Oct 2022 02:53
URI: https://ueaeprints.uea.ac.uk/id/eprint/64114
DOI: 10.1242/dev.147942

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