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Hintze, Mark, Singh Prajapati, Ravindra, Tambalo, Monica, Christophorou, Nicolas, Anwar, Maryam, Grocott, Timothy 
ORCID: https://orcid.org/0000-0002-6321-401X and Streit, Andrea
(2017)
Cell interactions, signals and transcriptional hierarchy governing placode progenitor induction.
Development, 144 (15).
pp. 2810-2823.
ISSN 0950-1991
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Abstract
In vertebrates, cranial placodes contribute to all sense organs and sensory ganglia and arsise from a common pool of Six1/Eya2+ progenitors. Here we dissect the events that specify ectodermal cells as placode progenitors using newly identified genes upstream of the Six/Eya complex. We show that two different tissues, the lateral head mesoderm and the prechordal mesendoderm, gradually induce placode progenitors: cells pass through successive transcriptional states, each identified by distinct factors and controlled by different signals. Both tissues initiate a common transcriptional state, but over time impart regional character and the acquisition of anterior identity depends on Shh signalling. Using a network inference approach we predict the regulatory relationships among newly identified transcription factors and verify predicted links using knock-down experiments. Based on this analysis we propose a new model for placode progenitor induction, in which the initial induction of a generic transcriptional state precedes regional divergence.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | chick embryo,cell fate,sense organs,sensory ganglia,transcriptional networks, signalling |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Cells and Tissues |
| Depositing User: | Pure Connector |
| Date Deposited: | 14 Jul 2017 05:06 |
| Last Modified: | 19 Apr 2023 22:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/64114 |
| DOI: | 10.1242/dev.147942 |
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