Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint

Davidson, Rose ORCID: https://orcid.org/0000-0002-6624-4011, Gardner, Sarah, Jupp, Orla, Bullough, Angela, Butters, Sue, Watts, Laura, Donell, Simon, Traka, Maria, Saha, Shikha, Mithen, Richard, Jeffers, Mandy, Clegg, Peter, Bao, Yongping ORCID: https://orcid.org/0000-0002-6425-0370, Cassidy, Aedin and Clark, Ian (2017) Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint. Scientific Reports, 7. ISSN 2045-2322

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Abstract

Osteoarthritis is a major cause of disability and there is no current pharmaceutical treatment which can prevent the disease or slow its progression. Dietary advice or supplementation is clearly an attractive option since it has low toxicity and ease of implementation on a population level. We have previously demonstrated that sulforaphane, a dietary isothiocyanate derived from its glucosinolate precursor which is found in broccoli, can prevent cartilage destruction in cells, in in vitro and in vivo models of osteoarthritis. As the next phase of this research, we enrolled 40 patients with knee osteoarthritis undergoing total knee replacement into a proof-of-principle trial. Patients were randomised to either a low or high glucosinolate diet for 14 days prior to surgery. We detected ITCs in the synovial fluid of the high glucosinolate group, but not the low glucosinolate group. This was mirrored by an increase in ITCs and specifically sulforaphane in the plasma. Proteomic analysis of synovial fluid showed significantly distinct profiles between groups with 125 differentially expressed proteins. The functional consequence of this diet will now be tested in a clinical trial.

Item Type:	Article
Uncontrolled Keywords:	osteoarthritis,sulforaphane,isothiocyanate,broccoli
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies Faculty of Medicine and Health Sciences > Research Groups > Cardiovascular and Metabolic Health Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	https://www.nature.com/articles/s41598-0...
Depositing User:	Pure Connector
Date Deposited:	14 Jun 2017 05:06
Last Modified:	25 Sep 2024 12:45
URI:	https://ueaeprints.uea.ac.uk/id/eprint/63742
DOI:	10.1038/s41598-017-03629-5

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