Mechanisms of iron uptake from ferric phosphate nanoparticles in human intestinal Caco-2 cells

Perfecto, Antonio, Elgy, Christine, Valsami-Jones, Eugenia, Sharp, Paul, Hilty, Florentine and Fairweather-Tait, Susan ORCID: https://orcid.org/0000-0002-1413-5569 (2017) Mechanisms of iron uptake from ferric phosphate nanoparticles in human intestinal Caco-2 cells. Nutrients, 9 (4). ISSN 2072-6643

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Abstract

Food fortification programs to reduce iron deficiency anemia require bioavailable forms of iron that do not cause adverse organoleptic effects. Rodent studies show that nano-sized ferric phosphate (NP-FePO4) is as bioavailable as ferrous sulfate, but there is controversy over the mechanism of absorption. We undertook in vitro studies to examine this using a Caco-2 cell model and simulated gastrointestinal (GI) digestion. Supernatant iron concentrations increased inversely with pH, and iron uptake into Caco-2 cells was 2–3 fold higher when NP-FePO4 was digested at pH 1 compared to pH 2. The size and distribution of NP-FePO4 particles during GI digestion was examined using transmission electron microscopy. The d50 of the particle distribution was 413 nm. Using disc centrifugal sedimentation, a high degree of agglomeration in NP-FePO4 following simulated GI digestion was observed, with only 20% of the particles ≤1000 nm. In Caco-2 cells, divalent metal transporter-1 (DMT1) and endocytosis inhibitors demonstrated that NP-FePO4 was mainly absorbed via DMT1. Small particles may be absorbed by clathrin-mediated endocytosis and micropinocytosis. These findings should be considered when assessing the potential of iron nanoparticles for food fortification

Item Type:	Article
Uncontrolled Keywords:	nano iron,np-fepo4,bioavailability,caco-2 cells,simulated gastrointestinal digestion,dmt1,endocytosis
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health
Depositing User:	Pure Connector
Date Deposited:	07 Apr 2017 05:09
Last Modified:	19 Oct 2023 01:58
URI:	https://ueaeprints.uea.ac.uk/id/eprint/63194
DOI:	10.3390/nu9040359

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