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ToolsCardin, V., Peña-Segura, C and Pasantes-Morales, H (1999) Activation and inactivation of taurine efflux in hyposmotic and isosmotic swelling in cortical astrocytes:Role of ionic strength and cell volume decrease. Journal of Neuroscience Research, 56 (6). pp. 659-667. ISSN 0360-4012
Full text not available from this repository. (Request a copy)Abstract
A decrease in intracellular ionic strength appears involved in the activation of swelling-elicited 3H-taurine efflux in cortical cultured astrocytes. Hyposmotic (50%) or isosmotic urea-induced swelling leading to a decrease of intracellular ionic strength, activated 3H-taurine efflux from a rate constant of about 0.008 min(-1) to 0.33 min(-1) (hyposmotic) and 0.59 min(-1) (urea). This efflux rate was markedly lower (maximal 0.03 min(-1)) in isosmotic swelling caused by K+ accumulation, where there is no decrease in ionic strength, or in cold (10 degrees C) hyposmotic medium (maximal 0.18 min(-1)), where swelling is reduced and consequently intracellular ionic strength is less affected. Also, astrocytes pretreated with hyperosmotic medium, which recover cell volume by ion accumulation, did not release 3H-taurine when they swelled by switching to isosmotic medium, but when volume was recovered by accumulation of urea, taurine release was restored. These results point to a key role of ionic strength in the activation of osmosensitive 3H-taurine efflux. In contrast, its inactivation was independent of the change in ionic strength but appears related to the reduction in cell volume after swelling, since despite the extent or direction of the change in ionic strength, the 3H-taurine efflux did not inactivate in isosmotic KCl-elicited swelling when cell volume did not recover nor in hyposmotic swelling when RVD was impaired by replacing NaCl in the medium by permeant osmolytes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | volume regulation,osmolytes,isosmotic swelling,hyposmotic swelling,urea,kcl swelling |
| Faculty \ School: | Faculty of Social Sciences > School of Psychology |
| Depositing User: | Pure Connector |
| Date Deposited: | 06 Apr 2017 07:00 |
| Last Modified: | 22 Oct 2022 02:32 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/63181 |
| DOI: | 10.1002/(SICI)1097-4547(19990615)56:6<659::AID-JNR12>3.0.CO;2-W |
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