The metabolic impact of extracellular nitrite on aerobic metabolism of Paracoccus denitrificans

Hartop, Katherine, Sullivan, Matthew ORCID:, Giannopoulos, George, Gates, Andrew ORCID:, Bond, Philip, Yuan, Zhiguo, Clarke, Thomas ORCID:, Rowley, Gary and Richardson, David ORCID: (2017) The metabolic impact of extracellular nitrite on aerobic metabolism of Paracoccus denitrificans. Water Research, 113. 207–214. ISSN 0043-1354

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Nitrite, in equilibrium with free nitrous acid (FNA), can inhibit both aerobic and anaerobic growth of microbial communities through bactericidal activities that have considerable potential for control of microbial growth in a range of water systems. There has been much focus on the effect of nitrite / FNA on anaerobic metabolism and so, to enhance understanding of the metabolic impact of nitrite / FNA on aerobic metabolism, a study was undertaken with a model denitrifying bacterium Paracoccus denitrificans PD1222. Extracellular nitrite inhibits aerobic growth of P. denitrificans in a pH dependent manner that is likely to be a result of both nitrite and free nitrous acid (FNA) (pKa = 3.25) and subsequent reactive nitrogen oxides generated from the intracellular passage of FNA into P. denitrificans. Increased expression of a gene encoding a flavohemoglobin protein (Fhp) (Pden_1689) was observed in response to extracellular nitrite. Construction and analysis of a deletion mutant established the Fhp to be involved in endowing nitrite / FNA resistance at high extracellular nitrite concentrations. Global transcriptional analysis confirmed nitrite-dependent expression of fhp and indicated that P. denitrificans expressed a number of stress response systems associated with protein, DNA and lipid repair. It is therefore suggested that nitrite causes a pH-dependent stress response that is due to the production of associated reactive nitrogen species, such as NO from the internalisation of FNA.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences

Faculty of Science > School of Natural Sciences
Depositing User: Pure Connector
Date Deposited: 15 Feb 2017 02:22
Last Modified: 13 May 2023 00:01
DOI: 10.1016/j.watres.2017.02.011

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