The metabolic impact of extracellular nitrite on aerobic metabolism of Paracoccus denitrificans

Hartop, Katherine, Sullivan, Matthew ORCID: https://orcid.org/0000-0003-2276-3132, Giannopoulos, George, Gates, Andrew ORCID: https://orcid.org/0000-0002-4594-5038, Bond, Philip, Yuan, Zhiguo, Clarke, Thomas ORCID: https://orcid.org/0000-0002-6234-1914, Rowley, Gary ORCID: https://orcid.org/0000-0002-5421-4333 and Richardson, David ORCID: https://orcid.org/0000-0002-6847-1832 (2017) The metabolic impact of extracellular nitrite on aerobic metabolism of Paracoccus denitrificans. Water Research, 113. 207–214. ISSN 0043-1354

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Abstract

Nitrite, in equilibrium with free nitrous acid (FNA), can inhibit both aerobic and anaerobic growth of microbial communities through bactericidal activities that have considerable potential for control of microbial growth in a range of water systems. There has been much focus on the effect of nitrite / FNA on anaerobic metabolism and so, to enhance understanding of the metabolic impact of nitrite / FNA on aerobic metabolism, a study was undertaken with a model denitrifying bacterium Paracoccus denitrificans PD1222. Extracellular nitrite inhibits aerobic growth of P. denitrificans in a pH dependent manner that is likely to be a result of both nitrite and free nitrous acid (FNA) (pKa = 3.25) and subsequent reactive nitrogen oxides generated from the intracellular passage of FNA into P. denitrificans. Increased expression of a gene encoding a flavohemoglobin protein (Fhp) (Pden_1689) was observed in response to extracellular nitrite. Construction and analysis of a deletion mutant established the Fhp to be involved in endowing nitrite / FNA resistance at high extracellular nitrite concentrations. Global transcriptional analysis confirmed nitrite-dependent expression of fhp and indicated that P. denitrificans expressed a number of stress response systems associated with protein, DNA and lipid repair. It is therefore suggested that nitrite causes a pH-dependent stress response that is due to the production of associated reactive nitrogen species, such as NO from the internalisation of FNA.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Natural Sciences (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Energy Materials Laboratory Faculty of Medicine and Health Sciences > Research Groups > Pathogen Biology Group
Depositing User:	Pure Connector
Date Deposited:	15 Feb 2017 02:22
Last Modified:	25 Sep 2024 12:36
URI:	https://ueaeprints.uea.ac.uk/id/eprint/62603
DOI:	10.1016/j.watres.2017.02.011

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