Molecular electrometer and binding of cations to phospholipid bilayers

Catte, Andrea, Girych, Mykhailo, Javanainen, Matti, Loison, Claire, Melcr, Josef, Miettinen, Markus S., Monticelli, Luca, Määttä, Jukka, Oganesyan, Vasily S. ORCID: https://orcid.org/0000-0002-8738-1146, Ollila, O. H. Samuli, Tynkkynen, Joona and Vilov, Sergey (2016) Molecular electrometer and binding of cations to phospholipid bilayers. Physical Chemistry Chemical Physics, 18. pp. 32560-32569. ISSN 1463-9076

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Abstract

Despite the vast amount of experimental and theoretical studies on the binding affinity of cations – especially the biologically relevant Na+ and Ca2+ – for phospholipid bilayers, there is no consensus in the literature. Here we show that by interpreting changes in the choline headgroup order parameters according to the ‘molecular electrometer’ concept [Seelig et al., Biochemistry, 1987, 26, 7535], one can directly compare the ion binding affinities between simulations and experiments. Our findings strongly support the view that in contrast to Ca2+ and other multivalent ions, Na+ and other monovalent ions (except Li+) do not specifically bind to phosphatidylcholine lipid bilayers at sub-molar concentrations. However, the Na+ binding affinity was overestimated by several molecular dynamics simulation models, resulting in artificially positively charged bilayers and exaggerated structural effects in the lipid headgroups. While qualitatively correct headgroup order parameter response was observed with Ca2+ binding in all the tested models, no model had sufficient quantitative accuracy to interpret the Ca2+:lipid stoichiometry or the induced atomistic resolution structural changes. All scientific contributions to this open collaboration work were made publicly, using nmrlipids.blogspot.fi as the main communication platform.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Chemistry (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Centre for Photonics and Quantum Science
Depositing User:	Pure Connector
Date Deposited:	02 Dec 2016 00:01
Last Modified:	03 Nov 2024 00:41
URI:	https://ueaeprints.uea.ac.uk/id/eprint/61576
DOI:	10.1039/c6cp04883h

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