The use of polyacrylamide hydrogels to study the effects of matrix stiffness on nuclear envelope properties

Minaisah, Rose Marie, Cox, Susan and Warren, Derek T. (2016) The use of polyacrylamide hydrogels to study the effects of matrix stiffness on nuclear envelope properties. In: Methods in Molecular Biology. Methods in Molecular Biology, 1411 . Springer, pp. 233-239. ISBN 978-1-4939-3528-4

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Abstract

Matrix-derived mechanical cues influence cell proliferation, motility, and differentiation. Recent findings clearly demonstrate that the nuclear envelope (NE) adapts and remodels in response to mechanical signals, including matrix stiffness, yet a plethora of studies have been performed on tissue culture plastic or glass that have a similar stiffness to cortical bone. Using methods that allow modulation of matrix stiffness will provide further insight into the role of the NE in physiological conditions and the impact of changes in stiffness observed during ageing and disease on cellular function. In this chapter, we describe the polyacrylamide hydrogel system, which allows fabrication of hydrogels with variable stiffness to better mimic the environment experienced by cells in most tissues of the body.

Item Type:	Book Section
Uncontrolled Keywords:	extracellular matrix and stiffness,hydrogels,mechanotransduction,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School:	Faculty of Science > School of Pharmacy (former - to 2024) Faculty of Social Sciences > School of Education and Lifelong Learning
UEA Research Groups:	Faculty of Science > Research Groups > Molecular and Tissue Pharmacology
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	Pure Connector
Date Deposited:	23 Nov 2016 04:05
Last Modified:	24 Sep 2024 08:05
URI:	https://ueaeprints.uea.ac.uk/id/eprint/61462
DOI:	10.1007/978-1-4939-3530-7_15

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