Trends in thermostability provide information on the nature of substrate, inhibitor, and lipid interactions with mitochondrial carriers

Crichton, Paul G. ORCID: https://orcid.org/0000-0003-3786-8359, Lee, Yang, Ruprecht, Jonathan J., Cerson, Elizabeth, Thangaratnarajah, Chancievan, King, Martin S. and Kunji, Edmund R. S. (2015) Trends in thermostability provide information on the nature of substrate, inhibitor, and lipid interactions with mitochondrial carriers. Journal of Biological Chemistry, 290 (13). pp. 8206-8217. ISSN 0021-9258

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Abstract

Background: Methods for rapid assessment of interactions of small molecules with membrane proteins in detergent are lacking.  Results: Thermostability measurements of mitochondrial transporters display informative trends about detergent, lipid, substrate, and inhibitor interactions.  Conclusion: Mechanistic insights are obtained by studying the thermostability of mitochondrial transporters.  Significance: Information about the nature of compound interactions with membrane proteins can be obtained rapidly.

Item Type:	Article
Uncontrolled Keywords:	membrane protein,mitochondrial transport,protein stability,transporter,uncoupling protein,adenine nucleotide translocase,detergent micelle,differential scanning fluorimetry,thermofluor assay
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Cardiovascular and Metabolic Health Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	http://www.jbc.org/content/290/13/8206
Depositing User:	Pure Connector
Date Deposited:	03 Nov 2016 12:00
Last Modified:	19 Oct 2023 01:51
URI:	https://ueaeprints.uea.ac.uk/id/eprint/61226
DOI:	10.1074/jbc.M114.616607

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