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Crichton, Paul G. 
ORCID: https://orcid.org/0000-0003-3786-8359, Affourtit, Charles, Albury, Mary S., Carré, Jane E. and Moore, Anthony L.
(2005)
Constitutive activity of Sauromatum guttatum alternative oxidase in Schizosaccharomyces pombe implicates residues in addition to conserved cysteines in α-keto acid activation.
FEBS Letters, 579 (2).
pp. 331-336.
ISSN 0014-5793
Abstract
Activity of the plant mitochondrial alternative oxidase (AOX) can be regulated by organic acids, notably pyruvate. To date, only two well-conserved cysteine residues have been implicated in this process. We report the functional expression of two AOX isozymes (Sauromatum guttatum Sg-AOX and Arabidopsis thaliana At-AOX1a) in Schizosaccharomyces pombe. Comparison of the response of these two isozymes to pyruvate in isolated yeast mitochondria and disrupted mitochondrial membranes reveals that in contrast to At-AOX1a, Sg-AOX activity is insensitive to pyruvate and appears to be in a constitutively active state. As both of these isozymes conserve the two cysteines, we propose that such contrasting behaviour must be a direct result of differences in their amino acid sequence and have subsequently identified novel candidate residues.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ccp,carbonyl cyanide m-chlorophenylhydrazone,q(h2),ubiquinol,alternative oxidase structure,regulation,pyruvate,plant respiration,s. pombe mitochondria |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Cardiovascular and Metabolic Health Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 03 Nov 2016 10:00 |
| Last Modified: | 19 Oct 2023 01:51 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/61212 |
| DOI: | 10.1016/j.febslet.2004.10.107 |
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