Constitutive activity of Sauromatum guttatum alternative oxidase in Schizosaccharomyces pombe implicates residues in addition to conserved cysteines in α-keto acid activation

Crichton, Paul G., Affourtit, Charles, Albury, Mary S., Carré, Jane E. and Moore, Anthony L. (2005) Constitutive activity of Sauromatum guttatum alternative oxidase in Schizosaccharomyces pombe implicates residues in addition to conserved cysteines in α-keto acid activation. FEBS Letters, 579 (2). pp. 331-336. ISSN 0014-5793

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Abstract

Activity of the plant mitochondrial alternative oxidase (AOX) can be regulated by organic acids, notably pyruvate. To date, only two well-conserved cysteine residues have been implicated in this process. We report the functional expression of two AOX isozymes (Sauromatum guttatum Sg-AOX and Arabidopsis thaliana At-AOX1a) in Schizosaccharomyces pombe. Comparison of the response of these two isozymes to pyruvate in isolated yeast mitochondria and disrupted mitochondrial membranes reveals that in contrast to At-AOX1a, Sg-AOX activity is insensitive to pyruvate and appears to be in a constitutively active state. As both of these isozymes conserve the two cysteines, we propose that such contrasting behaviour must be a direct result of differences in their amino acid sequence and have subsequently identified novel candidate residues.

Item Type: Article
Uncontrolled Keywords: ccp,carbonyl cyanide m-chlorophenylhydrazone,q(h2),ubiquinol,alternative oxidase structure,regulation,pyruvate,plant respiration,s. pombe mitochondria
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: Pure Connector
Date Deposited: 03 Nov 2016 10:00
Last Modified: 22 Apr 2020 01:54
URI: https://ueaeprints.uea.ac.uk/id/eprint/61212
DOI: 10.1016/j.febslet.2004.10.107

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