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ToolsCrichton, Paul G., Affourtit, Charles, Albury, Mary S., Carré, Jane E. and Moore, Anthony L. (2005) Constitutive activity of Sauromatum guttatum alternative oxidase in Schizosaccharomyces pombe implicates residues in addition to conserved cysteines in α-keto acid activation. FEBS Letters, 579 (2). pp. 331-336. ISSN 0014-5793
Full text not available from this repository. (Request a copy)Abstract
Activity of the plant mitochondrial alternative oxidase (AOX) can be regulated by organic acids, notably pyruvate. To date, only two well-conserved cysteine residues have been implicated in this process. We report the functional expression of two AOX isozymes (Sauromatum guttatum Sg-AOX and Arabidopsis thaliana At-AOX1a) in Schizosaccharomyces pombe. Comparison of the response of these two isozymes to pyruvate in isolated yeast mitochondria and disrupted mitochondrial membranes reveals that in contrast to At-AOX1a, Sg-AOX activity is insensitive to pyruvate and appears to be in a constitutively active state. As both of these isozymes conserve the two cysteines, we propose that such contrasting behaviour must be a direct result of differences in their amino acid sequence and have subsequently identified novel candidate residues.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ccp,carbonyl cyanide m-chlorophenylhydrazone,q(h2),ubiquinol,alternative oxidase structure,regulation,pyruvate,plant respiration,s. pombe mitochondria |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Cardiovascular and Metabolic Health Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 03 Nov 2016 10:00 |
| Last Modified: | 06 Feb 2025 06:49 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/61212 |
| DOI: | 10.1016/j.febslet.2004.10.107 |
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