Single-cell genomics based on Raman sorting reveals novel carotenoid-containing bacteria in the Red Sea

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Song, Yizhi, Kaster, Anne-Kristin, Vollmers, John, Song, Yanqing, Davison, Paul A., Frentrup, Martinique, Preston, Gail M., Thompson, Ian P., Murrell, J. Colin, Yin, Huabing, Hunter, C. Neil and Huang, Wei E. (2017) Single-cell genomics based on Raman sorting reveals novel carotenoid-containing bacteria in the Red Sea. Microbial Biotechnology, 10 (1). 125–137. ISSN 1751-7915

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Abstract

Cell sorting coupled with single-cell genomics is a powerful tool to circumvent cultivation of microorganisms and reveal microbial ‘dark matter’. Single-cell Raman spectra (SCRSs) are label-free biochemical ‘fingerprints’ of individual cells, which can link the sorted cells to their phenotypic information and ecological functions. We employed a novel Raman-activated cell ejection (RACE) approach to sort single bacterial cells from a water sample in the Red Sea based on SCRS. Carotenoids are highly diverse pigments and play an important role in phototrophic bacteria, giving strong and distinctive Raman spectra. Here, we showed that individual carotenoid-containing cells from a Red Sea sample were isolated based on the characteristic SCRS. RACE-based single-cell genomics revealed putative novel functional genes related to carotenoid and isoprenoid biosynthesis, as well as previously unknown phototrophic microorganisms including an unculturable Cyanobacteria spp. The potential of Raman sorting coupled to single-cell genomics has been demonstrated.

Item Type: Article
Additional Information: © 2016 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Faculty \ School: Faculty of Science > School of Environmental Sciences
University of East Anglia Research Groups/Centres > Theme - ClimateUEA
UEA Research Groups: Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation
Faculty of Science > Research Groups > Environmental Biology
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Depositing User: Pure Connector
Date Deposited: 18 Oct 2016 10:00
Last Modified: 20 Mar 2023 14:41
URI: https://ueaeprints.uea.ac.uk/id/eprint/60948
DOI: 10.1111/1751-7915.12420

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