Photoreduction of Shewanella oneidensis extracellular cytochromes by organic chromophores and dye-sensitized TiO2

Ainsworth, Emma V., Lockwood, Colin W. J., White, Gaye F., Hwang, Ee Taek, Sakai, Tsubasa, Gross, Manuela A., Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832, Clarke, Thomas A. ORCID: https://orcid.org/0000-0002-6234-1914, Jeuken, Lars J. C., Reisner, Erwin and Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226 (2016) Photoreduction of Shewanella oneidensis extracellular cytochromes by organic chromophores and dye-sensitized TiO2. ChemBioChem, 17 (24). 2324–2333. ISSN 1439-4227

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Abstract

The transfer of photoenergized electrons from extracellular photosensitizers across a bacterial cell envelope to drive intracellular chemical transformations represents an attractive way to harness Nature’s catalytic machinery for solar to chemicals conversion. In Shewanella oneidensis MR-1 (MR-1) trans-outermembrane electron transfer is performed by the extracellular cytochromes MtrC and OmcA acting together with the outermembrane spanning porin:cytochrome complex (MtrAB). Here we demonstrate photoreduction of solutions of MtrC, OmcA and the MtrCAB complex by soluble photosensitizers, namely, eosin Y, fluorescein, proflavine, flavin adenine dinucleotide and two compounds secreted by MR-1, riboflavin and flavin mononucleotide. We show photoreduction of MtrC and OmcA adsorbed on Ru(II)-dye sensitized TiO2 nanoparticles, and, that these protein coated particles perform photocatalytic reduction of solutions of MtrC, OmcA and MtrCAB. These findings provide a framework for informed development of strategies to use the outermembrane associated cytochromes of MR-1 for solar microbial synthesis in natural and engineered bacteria.

Item Type:	Article
Additional Information:	© 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords:	cytochromes,electron transfer,heme proteins,photocatalysis,photoreduction
Faculty \ School:	Faculty of Science > School of Chemistry Faculty of Science Faculty of Science > School of Biological Sciences Faculty of Science > School of Natural Sciences
Depositing User:	Pure Connector
Date Deposited:	29 Sep 2016 12:00
Last Modified:	12 May 2023 00:19
URI:	https://ueaeprints.uea.ac.uk/id/eprint/60632
DOI:	10.1002/cbic.201600339

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