Human IFIT1 inhibits mRNA translation of rubulaviruses but not other members of the Paramyxoviridae family

Young, D. F., Andrejeva, J., Li, X., Inesta-Vaquera, F., Dong, C., Cowling, V. H., Goodbourn, S. and Randall, R. E. (2016) Human IFIT1 inhibits mRNA translation of rubulaviruses but not other members of the Paramyxoviridae family. Journal of Virology, 90 (20). pp. 9446-9456. ISSN 0022-538X

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Abstract

We have previously shown that IFIT1 is primarily responsible for the antiviral action of interferon (IFN) alpha/beta against parainfluenza virus (PIV) type 5, selectively inhibiting the translation of PIV5 mRNAs. Here we report that whilst PIV2, PIV5 and mumps virus (MuV) are sensitive to IFIT1, non-rubulavirus members of the paramyxoviridae such as PIV3, Sendai virus (SeV) and canine distemper virus (CDV) are resistant. The IFIT1-sensitivity of PIV5 was not rescued by co-infection with an IFIT1-resistant virus (PIV3), demonstrating that PIV3 does not specifically inhibit the antiviral activity of IFIT1 and that the inhibition of PIV5 mRNAs is regulated by cis-acting elements. We developed an in vitro translation system using purified human IFIT1 to further investigate the mechanism of action of IFIT1. Whilst the translation of PIV2, PIV5 and MuV mRNAs were directly inhibited by IFIT1, the translation of PIV3, SeV and CDV mRNAs were not. Using purified human mRNA capping enzymes we show biochemically that efficient inhibition by IFIT1 is dependent upon a 5’ guanosine nucleoside cap (which need not be N7-methylated) and that this sensitivity is partly abrogated by 2’ O methylation of the cap 1 ribose. Intriguingly, PIV5 M mRNA, in contrast to NP mRNA, remained sensitive to inhibition by IFIT1 following in vitro 2’ O methylation, suggesting that other structural features of mRNAs may influence their sensitivity to IFIT1. Thus, surprisingly, the viral polymerases (which have 2’ -O-methyltransferase activity) of rubulaviruses do not protect these viruses from inhibition by IFIT1. Possible biological consequences of this are discussed.

Item Type:	Article
Additional Information:	Copyright © 2016 Young et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Related URLs:	http://jvi.asm.org/lookup/doi/10.1128/JV...
Depositing User:	Pure Connector
Date Deposited:	27 Sep 2016 10:00
Last Modified:	21 Mar 2024 01:26
URI:	https://ueaeprints.uea.ac.uk/id/eprint/60605
DOI:	10.1128/JVI.01056-16

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