Winfield, Mark O, Allen, Alexandra M, Burridge, Amanda J, Barker, Gary L A, Benbow, Harriet R, Wilkinson, Paul A, Coghill, Jane, Waterfall, Christy, Davassi, Alessandro, Scopes, Geoff, Pirani, Ali, Webster, Teresa, Brew, Fiona, Bloor, Claire, King, Julie, West, Claire ORCID: https://orcid.org/0000-0001-7649-9600, Griffiths, Simon, King, Ian, Bentley, Alison R and Edwards, Keith J
(2016)
High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool.
Plant Biotechnology Journal, 14 (5).
pp. 1195-206.
ISSN 1467-7644
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Abstract
In wheat, a lack of genetic diversity between breeding lines has been recognized as a significant block to future yield increases. Species belonging to bread wheat's secondary and tertiary gene pools harbour a much greater level of genetic variability, and are an important source of genes to broaden its genetic base. Introgression of novel genes from progenitors and related species has been widely employed to improve the agronomic characteristics of hexaploid wheat, but this approach has been hampered by a lack of markers that can be used to track introduced chromosome segments. Here, we describe the identification of a large number of single nucleotide polymorphisms that can be used to genotype hexaploid wheat and to identify and track introgressions from a variety of sources. We have validated these markers using an ultra-high-density Axiom(®) genotyping array to characterize a range of diploid, tetraploid and hexaploid wheat accessions and wheat relatives. To facilitate the use of these, both the markers and the associated sequence and genotype information have been made available through an interactive web site.
Item Type: | Article |
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Additional Information: | © 2015 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Uncontrolled Keywords: | genotyping array,next-generation sequencing,secondary and tertiary gene pools,single nucleotide polymorphism,wheat,wheat progenitors |
Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Population Health |
Depositing User: | Pure Connector |
Date Deposited: | 24 Sep 2016 00:42 |
Last Modified: | 06 Jun 2024 14:56 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/60192 |
DOI: | 10.1111/pbi.12485 |
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