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Giraud, Gerard, Schulze, Holger, Bachmann, Till T., Campbell, Colin J., Mount, Andrew R., Ghazal, Peter, Khondoker, Mizanur R. 
ORCID: https://orcid.org/0000-0002-1801-1635, Ember, Stuart W.J., Ciani, Ilenia, Tlili, Chaker, Walton, Anthony J., Terry, Jonathan G. and Crain, Jason
(2010)
Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates.
Chemical Physics Letters, 484 (4-6).
pp. 309-314.
ISSN 0009-2614
Abstract
In this Letter, we demonstrate the application of time-resolved fluorescence anisotropy measurements to detect solution state hybridization of streptavidin conjugate (CdSe)ZnS quantum dots (QD). The study was performed on samples containing 10 nM QD incubated with 800 nM DNA. We show that the rotational correlation time of QD–DNA constructs increases significantly upon hybridization with values of 330 ns (QD-ssDNA) and 1.3 μs (QD-dsDNA), corresponding to a diameter of 14 nm and 23 nm respectively. The present study opens a new modality for hybridization detection using quantum dots.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Epidemiology and Public Health Faculty of Medicine and Health Sciences > Research Groups > Public Health and Health Services Research (former - to 2023) Faculty of Science > Research Groups > Norwich Epidemiology Centre Faculty of Medicine and Health Sciences > Research Groups > Norwich Epidemiology Centre Faculty of Medicine and Health Sciences > Research Centres > Population Health |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 24 Sep 2016 00:40 |
| Last Modified: | 19 Oct 2023 01:46 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/60177 |
| DOI: | 10.1016/j.cplett.2009.11.032 |
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