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Alsultan, Abdulrahman A, Aboulmagd, Elsayed, Evans, Benjamin A 
ORCID: https://orcid.org/0000-0001-6849-9758 and Amyes, Sebastian G B
(2014)
Clonal diversity of Acinetobacter baumannii from diabetic patients in Saudi Arabian hospitals.
Journal of Medical Microbiology, 63.
pp. 1460-1466.
ISSN 0022-2615
Abstract
Carbapenem-resistant Acinetobacter baumannii (CR-AB) represents a major health-care problem, causing high rates of morbidity and mortality. This study investigated the clonality of CR-AB isolated from diabetic patients from different regions in Saudi Arabia, as well as the relatedness of the β-lactamase genes. A total of 64 non-repetitive CR-AB clinical isolates were collected from 16 different regions in Saudi Arabia from intensive care patients. Isolates were identified phenotypically by the Vitek 2 compact system and genotypically by amplification of the blaOXA-51-like gene. The target sequences were amplified by PCR and the clonal diversity of the isolates was explored by PFGE. Resistance studies revealed that the prevalence of imipenem and meropenem resistance was 92% and 96%, respectively, while the vast majority of the isolates were susceptible to tigecycline and colistin. In addition, blaVIM and blaOXA-23 were the most prevalent genes in the isolates under investigation, while ISAba1 was the most dominant insertion sequence. PFGE results showed 13 clusters; clone H was dominant, comprising 20 isolates from four hospitals, followed by clones C and F, comprising 11 isolates each from three and six hospitals, respectively. Moreover, the current study signified the clonal diversity of CR-AB in Saudi Arabia and showed the ability of some clones to infect patients in many different cities.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | acinetobacter infections,acinetobacter baumannii,anti-bacterial agents,carbapenems,diabetes mellitus,drug resistance, bacterial,electrophoresis, gel, pulsed-field,female,genetic variation,genotype,humans,male,saudi arabia,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health Faculty of Medicine and Health Sciences > Research Groups > Pathogen Biology Group |
| Depositing User: | Pure Connector |
| Date Deposited: | 24 Sep 2016 00:09 |
| Last Modified: | 25 Sep 2024 12:07 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/59877 |
| DOI: | 10.1099/jmm.0.079640-0 |
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