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Evans, B A 
ORCID: https://orcid.org/0000-0001-6849-9758, Hamouda, A, Towner, K J and Amyes, S G B
(2010)
Novel genetic context of multiple bla OXA-58 genes in Acinetobacter genospecies 3.
Journal of Antimicrobial Chemotherapy, 65 (8).
pp. 1586-1588.
ISSN 0305-7453
Abstract
Objectives: The detection in Acinetobacter genospecies 3 isolates of OXA-type carbapenemases, resulting in reduced susceptibility to carbapenem antibiotics, is increasingly reported. We identified an Acinetobacter genospecies 3 isolate carrying the gene for OXA-58 and aimed to resolve the genetic environment surrounding the blaOXA-58 gene. Methods: Species identification was confirmed by 16S-23S rRNA restriction analysis. MICs of imipenem, meropenem and ertapenem were determined, and the isolate was screened by PCR for blaOXA-23-like, blaOXA-40-like, blaOXA-51-like and blaOXA-58-like genes. The sequence surrounding blaOXA-58 was determined through amplification by inverse PCR and genome walking followed by sequencing. Genetic localization was investigated by Southern blotting. Results: Isolate A164 was confirmed as belonging to Acinetobacter genospecies 3 and had reduced susceptibility to the carbapenems. The isolate was found to encode two blaOXA-58 genes that may have been duplicated by the insertion sequence ISAba125, two copies of which were inserted into ISAba3 elements. The blaOXA-58 genes appear to be plasmid borne. Conclusions: This is the first report of β-lactamase duplication in Acinetobacter genospecies 3 and of gene duplication mediated by ISAba125.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | acinetobacter spp,oxa-type β-lactamases,mobile elements,carbapenems |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health Faculty of Medicine and Health Sciences > Research Groups > Pathogen Biology Group |
| Depositing User: | Pure Connector |
| Date Deposited: | 24 Sep 2016 00:09 |
| Last Modified: | 25 Sep 2024 12:07 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/59871 |
| DOI: | 10.1093/jac/dkq180 |
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