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Lopes, Bruno S, Evans, Benjamin A 
ORCID: https://orcid.org/0000-0001-6849-9758 and Amyes, Sebastian G B
(2012)
Disruption of the blaOXA-51-like gene by ISAba16 and activation of the blaOXA-58 gene leading to carbapenem resistance in Acinetobacter baumannii Ab244.
Journal of Antimicrobial Chemotherapy, 67 (1).
pp. 59-63.
ISSN 0305-7453
Abstract
Objectives: This study examines the mechanism of carbapenem resistance in Acinetobacter baumannii isolate Ab244. Methods: A multiplex PCR for the detection of the blaOXA-23-like, blaOXA-40-like, blaOXA-51-like and blaOXA-58-like families was performed. MICs of imipenem and meropenem were determined by the agar dilution method. The sequence surrounding the blaOXA-132 gene was determined by amplification with primer pairs encompassing a part of fxsA and an acetyltransferase gene (GNAT). The sequence upstream of the blaOXA-58 gene was determined by sequencing. SDS–PAGE and carO PCR were performed to check the integrity of the outer membrane proteins. RT–PCRs for the expression of the blaOXA-132 gene and the blaOXA-58 gene were performed. Results: Isolate Ab244 harboured blaOXA-132 belonging to the blaOXA-51-like gene cluster and a blaOXA-58 gene. The 4239 bp region between fxsA and GNAT showed an insert of ISAba16 (where IS stands for insertion sequence) after the first 15 nucleotides of the blaOXA-132 gene, with an 8 bp target site duplication at the 5′ and 3′ ends of ISAba16. The sequence oriented in the 5′→3′ direction caused insertional inactivation of the blaOXA-132 gene. The blaOXA-58 gene was highly expressed by the promoters provided by an ISAba3-like structure found upstream of the gene. The isolate was resistant to meropenem and had intermediate resistance to imipenem, and was also positive for ISAba1. Conclusions: This is the first report showing ISAba16-mediated inactivation of the blaOXA-132 gene in strain Ab244. The resistance to carbapenems in strain Ab244 is related to the acquisition of the blaOXA-58 gene, here governed by an ISAba3-like element.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | a. baumannii,insertion sequences,β-lactamases,gene environment |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health Faculty of Medicine and Health Sciences > Research Groups > Pathogen Biology Group |
| Depositing User: | Pure Connector |
| Date Deposited: | 24 Sep 2016 00:09 |
| Last Modified: | 25 Sep 2024 12:06 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/59866 |
| DOI: | 10.1093/jac/dkr415 |
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