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Bidula, Stefan 
ORCID: https://orcid.org/0000-0002-3790-7138, Sexton, Darren W. and Schelenz, Silke
(2016)
Serum opsonin ficolin-A enhances host–fungal interactions and modulates cytokine expression from human monocyte-derived macrophages and neutrophils following Aspergillus fumigatus challenge.
Medical Microbiology and Immunology, 205 (2).
pp. 133-142.
ISSN 0300-8584
Abstract
Invasive aspergillosis is a devastating invasive fungal disease associated with a high mortality rate in the immunocompromised, such as leukaemia patients, transplant patients and those with HIV/AIDS. The rodent serum orthologue of human L-ficolin, ficolin-A, can bind to and opsonize Aspergillus fumigatus, the pathogen that causes invasive aspergillosis, and may participate in fungal defence. Using human monocyte-derived macrophages and neutrophils isolated from healthy donors, we investigated conidial association and fungal viability by flow cytometry and microscopy. Additionally, cytokine production was measured via cytometric bead arrays. Ficolin-A opsonization was observed to significantly enhance association of conidia, while also inhibiting hyphal growth and contributing to increased fungal killing following incubation with monocyte-derived macrophages and neutrophils. Additionally, ficolin-A opsonization was capable of manifesting a decrease in IL-8, IL-1β, IL-6, IL-10 and TNF-α production from MDM and IL-1β, IL-6 and TNF-α from neutrophils 24 h post-infection. In conclusion, rodent ficolin-A is functionally comparable to human L-ficolin and is capable of modulating the innate immune response to A. fumigatus, down-regulating cytokine production and could play an important role in airway immunity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | aspergillosis,macrophage,neutrophil,cytokines,innate immunity,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Norwich Clinical Trials Unit Faculty of Medicine and Health Sciences > Research Groups > Medical Microbiology (former - to 2018) Faculty of Medicine and Health Sciences > Research Groups > Pathogen Biology Group |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 01 Jul 2016 16:00 |
| Last Modified: | 25 Sep 2024 12:03 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/59647 |
| DOI: | 10.1007/s00430-015-0435-9 |
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