Isolation and fine mapping of Rps6: An intermediate host resistance gene in barley to wheat stripe rust

Dawson, Andrew M., Ferguson, John N., Gardiner, Matthew, Green, Phon, Hubbard, Amelia and Moscou, Matthew J. ORCID: https://orcid.org/0000-0003-2098-6818 (2016) Isolation and fine mapping of Rps6: An intermediate host resistance gene in barley to wheat stripe rust. Theoretical and Applied Genetics, 129 (4). pp. 831-843. ISSN 0040-5752

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Abstract

A plant may be considered a nonhost of a pathogen if all known genotypes of a plant species are resistant to all known isolates of a pathogen species. However, if a small number of genotypes are susceptible to some known isolates of a pathogen species this plant maybe considered an intermediate host. Barley (Hordeum vulgare) is an intermediate host for Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust. We wanted to understand the genetic architecture underlying resistance to Pst and to determine whether any overlap exists with resistance to the host pathogen, Puccinia striiformis f. sp. hordei (Psh). We mapped Pst resistance to chromosome 7H and show that host and intermediate host resistance is genetically uncoupled. Therefore, we designate this resistance locus Rps6. We used phenotypic and genotypic selection on F2:3 families to isolate Rps6 and fine mapped the locus to a 0.1 cM region. Anchoring of the Rps6 locus to the barley physical map placed the region on two adjacent fingerprinted contigs. Efforts are now underway to sequence the minimal tiling path and to delimit the physical region harbouring Rps6. This will facilitate additional marker development and permit identification of candidate genes in the region.

Item Type:	Article
Additional Information:	© The Author(s) 2016 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://​creativecommons.​org/​licenses/​by/​4.​0/​), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > The Sainsbury Laboratory
Depositing User:	Pure Connector
Date Deposited:	19 May 2016 11:00
Last Modified:	18 Oct 2024 23:43
URI:	https://ueaeprints.uea.ac.uk/id/eprint/58883
DOI:	10.1007/s00122-015-2659-x

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