Differential effects of EPA vs. DHA on postprandial vascular function and the plasma oxylipin profile in men

McManus, Sean, Tejera, Noemi, Awwad, Khader, Vauzour, David ORCID: https://orcid.org/0000-0001-5952-8756, Rigby, Neil, Fleming, Ingrid, Cassidy, Aedin and Minihane, Anne-Marie ORCID: https://orcid.org/0000-0001-9042-4226 (2016) Differential effects of EPA vs. DHA on postprandial vascular function and the plasma oxylipin profile in men. The Journal of Lipid Research, 57. pp. 1720-1727. ISSN 0022-2275

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Abstract

Our objective was to investigate the impact of EPA versus DHA, on arterial stiffness and reactivity, and underlying mechanisms (with a focus on plasma oxylipins), in the postprandial state. In a 3-arm cross-over acute test meal trial men (n=26, 35-55y) at increased CVD risk, received a high fat (42.4g) test meal providing 4.16 g of EPA or DHA or control oil in random order. At 0 h and 4 h, blood samples were collected to quantify plasma fatty acids, LCn-3PUFAs derived oxylipins, nitrite and hydrogen sulfide and serum lipids and glucose. Vascular function was assessed using blood pressure, Reactive Hyperaemia Index (RHI), Pulse Wave Velocity and Augmentation Index (AIx). The DHA-rich oil significantly reduced AIx by 13% (P=0.047) with the decrease following EPA-rich oil intervention not reaching statistical significance. Both interventions increased EPA and DHA derived oxylipins in the acute postprandial state, with an (1.3 fold) increase in 19,20-DiHDPA evident after DHA intervention (P < 0.001). In conclusion, a single dose of DHA significantly improved postprandial arterial stiffness as assessed by AIx, which if sustained would be associated with a significant decrease in CVD risk. The observed increases in oxylipins provide a mechanistic insight for the AIx effect.

Item Type:	Article
Additional Information:	Copyright © 2016 by the American Society for Biochemistry and Molecular Biology, Inc. Author’s Choice—Final version free via Creative Commons CC-BY license.
Uncontrolled Keywords:	augmentation index,blood pressure,fish oil,hydrogen sulfide,lipidomics,nitric oxide,nutrition,omega-3 fatty acids,pulse wave velocity
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Cardiovascular and Metabolic Health Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health
Depositing User:	Pure Connector
Date Deposited:	17 May 2016 12:00
Last Modified:	19 Oct 2023 01:42
URI:	https://ueaeprints.uea.ac.uk/id/eprint/58785
DOI:	10.1194/jlr.M067801

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