MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development

Giusti, Juliana, Pinhal, Danillo, Moxon, Simon ORCID: https://orcid.org/0000-0003-4644-1816, Lovaglio Campos, Camilla, Munsterberg, Andrea ORCID: https://orcid.org/0000-0002-4577-4240 and Martins, Cesar (2016) MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development. Mechanisms of Development, 140. pp. 12-18. ISSN 0925-4773

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Abstract

Hox gene clusters encode a family of transcription factors that govern anterior posterior axis patterning during embryogenesis in all bilaterian animals. The time and place ofHox gene expression are largely determined by the relative position of each gene within its cluster. Furthermore, Hox genes were shown to have their expression fine-tuned by regulatory microRNAs (miRNAs). However, the mechanisms of miRNA-mediated regulation of these transcription factors during fish early development remain largely unknown. Here we have profiled three highly expressed miR-10 family members of Nile tilapia at early embryonic development, determined their genomic organization as well as performed functional experiments for validation of target genes. Quantitative analysis during developmental stages showed miR-10 family expression negatively correlates with the expression of HoxA3a, HoxB3a and HoxD10a genes, as expected for bona fide miRNA–mRNA interactions. Moreover, luciferase assays demonstrated that HoxB3a and HoxD10a are targeted by miR-10b-5p. Overall, our data indicate that the miR-10 family directly regulates members of the Hox gene family during Nile tilapia embryogenesis.

Item Type:	Article
Uncontrolled Keywords:	oreochromis niloticus,embryos,mirna,hoxa3a,hoxb3a,hoxd10a
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Cells and Tissues
Depositing User:	Pure Connector
Date Deposited:	25 Apr 2016 10:00
Last Modified:	19 Apr 2023 23:55
URI:	https://ueaeprints.uea.ac.uk/id/eprint/58348
DOI:	10.1016/j.mod.2016.03.002

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