An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment

Webster, Carl, Di Silvio, Desire, Devarajan, Aarthi, Bigini, Paolo, Micotti, Eduardo, Giudice, Chiari, Salmona, Mario, Wheeler, Grant ORCID: https://orcid.org/0000-0002-4335-8577, Sherwood, Victoria and Baldelli Bombelli, Francesca (2016) An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment. Nanomedicine, 11 (6). pp. 643-656. ISSN 1743-5889

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Abstract

Background. With the rise in production of nanoparticles for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a nanoparticle hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index. Results. We observed that, after exposure to a range of nanoparticles, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored nanoparticles, negative for toxicity in vitro and Xenopus, were further confirmed as non-toxic in mice. Conclusion. The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of nanoparticles, which could be introduced for the routine testing of nanomaterials.

Item Type: Article
Uncontrolled Keywords: nanotoxicity,nanomaterials,xenopus laevis,nanomedicine,cytotoxicity,in vitro-in vivo correlations
Faculty \ School: Faculty of Science > School of Pharmacy
Faculty of Science > School of Biological Sciences
Faculty of Science
Depositing User: Pure Connector
Date Deposited: 05 Apr 2016 16:00
Last Modified: 22 Oct 2022 00:47
URI: https://ueaeprints.uea.ac.uk/id/eprint/58141
DOI: 10.2217/nnm.15.219

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