An early developmental vertebrate model for nanomaterial safety:bridging cell-based and mammalian toxicity assessment

Webster, Carl, Di Silvio, Desire, Devarajan, Aarthi, Bigini, Paolo, Micotti, Eduardo, Giudice, Chiari, Salmona, Mario, Wheeler, Grant, Sherwood, Victoria and Baldelli Bombelli, Francesca (2016) An early developmental vertebrate model for nanomaterial safety:bridging cell-based and mammalian toxicity assessment. Nanomedicine, 11 (6). pp. 643-656. ISSN 1743-5889

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Abstract

Background. With the rise in production of nanoparticles for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a nanoparticle hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index. Results. We observed that, after exposure to a range of nanoparticles, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored nanoparticles, negative for toxicity in vitro and Xenopus, were further confirmed as non-toxic in mice. Conclusion. The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of nanoparticles, which could be introduced for the routine testing of nanomaterials.

Item Type: Article
Uncontrolled Keywords: nanotoxicity,nanomaterials,xenopus laevis,nanomedicine,cytotoxicity,in vitro-in vivo correlations
Faculty \ School: Faculty of Science > School of Pharmacy
Faculty of Science > School of Biological Sciences
Faculty of Science
Depositing User: Pure Connector
Date Deposited: 05 Apr 2016 16:00
Last Modified: 24 Jun 2020 23:58
URI: https://ueaeprints.uea.ac.uk/id/eprint/58141
DOI: 10.2217/nnm.15.219

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