Lactobacillus reuteri inhibition of Enteropathogenic Escherichia coli adherence to human intestinal epithelium

Walsham, Alistair, MacKenzie, Donald A., Cook, Vivienne, Wemyss-Holden, Simon, Hews, Claire, Juge, Nathalie and Schuller, Stephanie ORCID: https://orcid.org/0000-0003-3260-9112 (2016) Lactobacillus reuteri inhibition of Enteropathogenic Escherichia coli adherence to human intestinal epithelium. Frontiers in Microbiology, 7 (244). ISSN 1664-302X

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Abstract

Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrheal infant death in developing countries, and probiotic bacteria have been shown to provide health benefits in gastrointestinal infections. In this study, we have investigated the influence of the gut symbiont Lactobacillus reuteri on EPEC adherence to the human intestinal epithelium. Different host cell model systems including non-mucus-producing HT-29 and mucus-producing LS174T intestinal epithelial cell lines as well as human small intestinal biopsies were used. Adherence of L. reuteri to HT-29 cells was strain-specific, and the mucus-binding proteins CmbA and MUB increased binding to both HT-29 and LS174T cells. L. reuteri ATCC PTA 6475 and ATCC 53608 significantly inhibited EPEC binding to HT-29 but not LS174T cells. While pre-incubation of LS174T cells with ATCC PTA 6475 did not affect EPEC attaching/effacing (A/E) lesion formation, it increased the size of EPEC microcolonies. ATCC PTA 6475 and ATCC 53608 binding to the mucus layer resulted in decreased EPEC adherence to small intestinal biopsy epithelium. Our findings show that L. reuteri reduction of EPEC adhesion is strain-specific and has the potential to target either the epithelium or the mucus layer, providing further rationale for the selection of probiotic strains.

Item Type:	Article
Additional Information:	© 2016 Walsham, MacKenzie, Cook, Wemyss-Holden, Hews, Juge and Schüller. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Uncontrolled Keywords:	l. reuteri,epec,diarrhea,probiotic,human intestinal epithelium,adherence,mucus,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	Pure Connector
Date Deposited:	22 Mar 2016 09:28
Last Modified:	19 Oct 2023 01:38
URI:	https://ueaeprints.uea.ac.uk/id/eprint/57778
DOI:	10.3389/fmicb.2016.00244

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