Validation of three rapid screening methods for detection of verotoxin-producing Escherichia coli in foods:interlaboratory study

Capps, Katherine L, McLaughlin, Emiline M, Murray, Alistair W A, Aldus, Clare F, Wyatt, Gary M, Peck, Michael W, van Amerongen, Aart, Ariëns, Renata M C, Wichers, Jan H, Baylis, Christopher L, Wareing, David R A and Bolton, Frederick J (2004) Validation of three rapid screening methods for detection of verotoxin-producing Escherichia coli in foods:interlaboratory study. Journal of AOAC International, 87 (1). pp. 68-77. ISSN 1060-3271

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Abstract

An interlaboratory study was conducted for the validation of 3 methods for the detection of all verotoxin-producing Escherichia coli (VTEC) in foods. The methods were a multi-analyte 1-step lateral flow immunoassay (LFIA) for detection of E. coli O157 and verotoxin (VT); an enzyme-linked immunosorbent assay targeted against VT1, VT2, and VT2c (VT-ELISA); and a polymerase chain reaction (PCR) method for detection of VT genes (VT-PCR). Aliquots (25 g or 25 mL) of 4 food types (raw minced [ground] beef, unpasteurized milk, unpasteurized apple juice [cider], and salami) were individually inoculated with low numbers (<9 to 375 cells/25 g) of 6 test strains of E. coli (serogroups O26, O103, O111, O145, and O157) with differing VT-producing capabilities. Five replicates for each test strain and 5 uninoculated samples were prepared for each food type. Fourteen participating laboratories analyzed samples using the LFIA, 9 analyzed the samples by ELISA, and 9 by PCR. The LFIA for O157 and VT had a specificity (correct identification of negative samples) of 92 and 94%, respectively, and a sensitivity (correct identification of positive samples) of 94 and 55%, respectively. The VT-ELISA and VT-PCR had a specificity of 98 and 99%, respectively, and a sensitivity of 89 and 72%, respectively.

Item Type: Article
Uncontrolled Keywords: animals,beverages,colony count, microbial,enzyme-linked immunosorbent assay,escherichia coli,escherichia coli o157,food microbiology,immunoassay,malus,meat,milk,reproducibility of results,reverse transcriptase polymerase chain reaction,shiga toxins
Faculty \ School: Faculty of Medicine and Health Sciences > School of Health Sciences
Faculty of Science > School of Biological Sciences
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Depositing User: Pure Connector
Date Deposited: 17 Mar 2016 15:00
Last Modified: 22 Apr 2020 01:11
URI: https://ueaeprints.uea.ac.uk/id/eprint/57564
DOI:

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