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Capps, Katherine L, McLaughlin, Emiline M, Murray, Alistair W A, Aldus, Clare F 
ORCID: https://orcid.org/0000-0002-0197-2755, Wyatt, Gary M, Peck, Michael W, van Amerongen, Aart, Ariëns, Renata M C, Wichers, Jan H, Baylis, Christopher L, Wareing, David R A and Bolton, Frederick J
(2004)
Validation of three rapid screening methods for detection of verotoxin-producing Escherichia coli in foods: interlaboratory study.
Journal of AOAC International, 87 (1).
pp. 68-77.
ISSN 1060-3271
Abstract
An interlaboratory study was conducted for the validation of 3 methods for the detection of all verotoxin-producing Escherichia coli (VTEC) in foods. The methods were a multi-analyte 1-step lateral flow immunoassay (LFIA) for detection of E. coli O157 and verotoxin (VT); an enzyme-linked immunosorbent assay targeted against VT1, VT2, and VT2c (VT-ELISA); and a polymerase chain reaction (PCR) method for detection of VT genes (VT-PCR). Aliquots (25 g or 25 mL) of 4 food types (raw minced [ground] beef, unpasteurized milk, unpasteurized apple juice [cider], and salami) were individually inoculated with low numbers (<9 to 375 cells/25 g) of 6 test strains of E. coli (serogroups O26, O103, O111, O145, and O157) with differing VT-producing capabilities. Five replicates for each test strain and 5 uninoculated samples were prepared for each food type. Fourteen participating laboratories analyzed samples using the LFIA, 9 analyzed the samples by ELISA, and 9 by PCR. The LFIA for O157 and VT had a specificity (correct identification of negative samples) of 92 and 94%, respectively, and a sensitivity (correct identification of positive samples) of 94 and 55%, respectively. The VT-ELISA and VT-PCR had a specificity of 98 and 99%, respectively, and a sensitivity of 89 and 72%, respectively.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | animals,beverages,colony count, microbial,enzyme-linked immunosorbent assay,escherichia coli,escherichia coli o157,food microbiology,immunoassay,malus,meat,milk,reproducibility of results,reverse transcriptase polymerase chain reaction,shiga toxins |
| Faculty \ School: | Faculty of Medicine and Health Sciences > School of Health Sciences Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Health Promotion Faculty of Science > Research Groups > Norwich Epidemiology Centre Faculty of Medicine and Health Sciences > Research Groups > Norwich Epidemiology Centre Faculty of Medicine and Health Sciences > Research Centres > Population Health |
| Depositing User: | Pure Connector |
| Date Deposited: | 17 Mar 2016 15:00 |
| Last Modified: | 06 Jun 2024 14:54 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/57564 |
| DOI: | 10.1093/jaoac/87.1.68 |
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