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Edwards, Marcus, White, Gaye, Norman, Michael, Tome-Fernandez, Alice, Ainsworth, Emma, Shi, Liang, Fredrickson, Jim, Zachara, John, Butt, Julea 
ORCID: https://orcid.org/0000-0002-9624-5226, Richardson, David ORCID: https://orcid.org/0000-0002-6847-1832 and Clarke, Tom ORCID: https://orcid.org/0000-0002-6234-1914
(2015)
Redox linked flavin sites in extracellular decaheme proteins involved in microbe-mineral electron transfer.
Scientific Reports, 5.
ISSN 2045-2322
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Abstract
Extracellular microbe-mineral electron transfer is a major driving force for the oxidation of organic carbon in many subsurface environments. Extracellular multi-heme cytochromes of the Shewenella genus play a major role in this process but the mechanism of electron exchange at the interface between cytochrome and acceptor is widely debated. The 1.8 Å x-ray crystal structure of the decaheme MtrC revealed a highly conserved CX8C disulfide that, when substituted for AX8A, severely compromised the ability of S. oneidensis to grow under aerobic conditions. Reductive cleavage of the disulfide in the presence of flavin mononucleotide (FMN) resulted in the reversible formation of a stable flavocytochrome. Similar results were also observed with other decaheme cytochromes, OmcA, MtrF and UndA. The data suggest that these decaheme cytochromes can transition between highly reactive flavocytochromes or less reactive cytochromes, and that this transition is controlled by a redox active disulfide that responds to the presence of oxygen.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | mtrc,cytochrome,flavin,shewanella,mineral respiration,metal reduction |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science Faculty of Science > School of Chemistry Faculty of Science > School of Natural Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Energy Materials Laboratory |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 17 Mar 2016 13:32 |
| Last Modified: | 09 Mar 2024 00:45 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/57548 |
| DOI: | 10.1038/srep11677 |
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