Altered expression of type-1 and type-2 cannabinoid receptors in celiac disease

Battista, Natalia, Di Sabatino, Antonio, Di Tommaso, Monia, Biancheri, Paolo, Rapino, Cinzia, Giuffrida, Paolo, Papadia, Cinzia, Montana, Chiara, Pasini, Alessandra, Vanoli, Alessandro, Lanzarotto, Francesco, Villanacci, Vincenzo, Corazza, Gino R. and Maccarrone, Mauro (2013) Altered expression of type-1 and type-2 cannabinoid receptors in celiac disease. PLoS One, 8 (4). ISSN 1932-6203

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Abstract

Anandamide (AEA) is the prominent member of the endocannabinoid family and its biological action is mediated through the binding to both type-1 (CB1) and type-2 (CB2) cannabinoid receptors (CBR). The presence of AEA and CBR in the gastrointestinal tract highlighted their pathophysiological role in several gut diseases, including celiac disease. Here, we aimed to investigate the expression of CBR at transcriptional and translational levels in the duodenal mucosa of untreated celiac patients, celiac patients on a gluten-free diet for at least 12 months and control subjects. Also biopsies from treated celiac patients cultured ex vivo with peptic-tryptic digest of gliadin were investigated. Our data show higher levels of both CB1 and CB2 receptors during active disease and normal CBR levels in treated celiac patients. In conclusion, we demonstrate an up-regulation of CB1 and CB2 mRNA and protein expression, that points to the therapeutic potential of targeting CBR in patients with celiac disease.

Item Type:	Article
Additional Information:	© 2013 Battista et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Uncontrolled Keywords:	adult,celiac disease,female,fluorescent antibody technique,gene expression regulation,gliadin,humans,intestinal mucosa,male,confocal microscopy,protein binding,messenger rna,cannabinoid receptor cb1 ,cannabinoid receptor cb2
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User:	Pure Connector
Date Deposited:	17 Mar 2016 12:01
Last Modified:	22 Oct 2022 00:54
URI:	https://ueaeprints.uea.ac.uk/id/eprint/57532
DOI:	10.1371/journal.pone.0062078

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