Novel methanotrophs of the family Methylococcaceae from different geographical regions and habitats

Islam, Tajul, Larsen, Øivind, Torsvik, Vigdis, Øvreås, Lise, Panosyan, Hovik, Murrell, J. Colin, Birkeland, Nils-Kåre and Bodrossy, Levente (2015) Novel methanotrophs of the family Methylococcaceae from different geographical regions and habitats. Microorganisms, 3 (3). pp. 484-499. ISSN 2076-2607

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Abstract

Terrestrial methane seeps and rice paddy fields are important ecosystems in the methane cycle. Methanotrophic bacteria in these ecosystems play a key role in reducing methane emission into the atmosphere. Here, we describe three novel methanotrophs, designated BRS-K6, GFS-K6 and AK-K6, which were recovered from three different habitats in contrasting geographic regions and ecosystems: waterlogged rice-field soil and methane seep pond sediments from Bangladesh; and warm spring sediments from Armenia. All isolates had a temperature range for growth of 8–35 °C (optimal 25–28 °C) and a pH range of 5.0–7.5 (optimal 6.4–7.0). 16S rRNA gene sequences showed that they were new gammaproteobacterial methanotrophs, which form a separate clade in the family Methylococcaceae. They fell into a cluster with thermotolerant and mesophilic growth tendency, comprising the genera Methylocaldum-Methylococcus-Methyloparacoccus-Methylogaea. So far, growth below 15 °C of methanotrophs from this cluster has not been reported. The strains possessed type I intracytoplasmic membranes. The genes pmoA, mxaF, cbbL, nifH were detected, but no mmoX gene was found. Each strain probably represents a novel species either belonging to the same novel genus or each may even represent separate genera. These isolates extend our knowledge of methanotrophic Gammaproteobacteria and their physiology and adaptation to different ecosystems.

Item Type:	Article
Uncontrolled Keywords:	gammaproteobacteria,methylococcaceae,methanotrophs,methylocaldum,mesophilic,terrestrial,sdg 15 - life on land ,/dk/atira/pure/sustainabledevelopmentgoals/life_on_land
Faculty \ School:	Faculty of Science > School of Environmental Sciences
UEA Research Groups:	Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation Faculty of Science > Research Groups > Environmental Biology Faculty of Science > Research Groups > Resources, Sustainability and Governance (former - to 2018) Faculty of Science > Research Groups > Marine and Atmospheric Sciences (former - to 2017)
Depositing User:	Pure Connector
Date Deposited:	15 Mar 2016 13:00
Last Modified:	04 Mar 2024 17:29
URI:	https://ueaeprints.uea.ac.uk/id/eprint/57487
DOI:	10.3390/microorganisms3030484

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