Targeted metagenomics of active microbial populations with stable-isotope probing

Coyotzi, Sara, Pratscher, Jennifer, Murrell, J. Colin and Neufeld, Josh D. (2016) Targeted metagenomics of active microbial populations with stable-isotope probing. Current Opinion in Biotechnology, 41. pp. 1-8. ISSN 0958-1669

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Abstract

The ability to explore microbial diversity and function has been enhanced by novel experimental and computational tools. The incorporation of stable isotopes into microbial biomass enables the recovery of labeled nucleic acids from active microorganisms, despite their initial abundance and culturability. Combining stable-isotope probing (SIP) with metagenomics provides access to genomes from microorganisms involved in metabolic processes of interest. Studies using metagenomic analysis on DNA obtained from DNA-SIP incubations can be ideal for the recovery of novel enzymes for biotechnology applications, including biodegradation, biotransformation, and biosynthesis. This chapter introduces metagenomic and DNA-SIP methodologies, highlights biotechnology-focused studies that combine these approaches, and provides perspectives on future uses of these methods as analysis tools for applied and environmental microbiology.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Environmental Sciences
UEA Research Groups:	Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation Faculty of Science > Research Groups > Environmental Biology Faculty of Science > Research Groups > Resources, Sustainability and Governance (former - to 2018) Faculty of Science > Research Groups > Marine and Atmospheric Sciences (former - to 2017)
Depositing User:	Pure Connector
Date Deposited:	15 Mar 2016 12:00
Last Modified:	21 Oct 2022 04:33
URI:	https://ueaeprints.uea.ac.uk/id/eprint/57480
DOI:	10.1016/j.copbio.2016.02.017

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