Expression of protocadherin gamma in skeletal muscle tissue is associated with age and muscle weakness

Hangelbroek, Roland W. J., Fazelzadeh, Parastoo, Tieland, Michael, Boekschoten, Mark V., Hooiveld, Guido J. E. J., van Duynhoven, John P. M., Timmons, James A., Verdijk, Lex B., de Groot, Lisette C. P. G. M., van Loon, Luc J. C. and Müller, Michael ORCID: https://orcid.org/0000-0002-5930-9905 (2016) Expression of protocadherin gamma in skeletal muscle tissue is associated with age and muscle weakness. Journal of Cachexia, Sarcopenia and Muscle, 7 (5). 604–614. ISSN 2190-5991

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Abstract

Background: The skeletal muscle system plays an important role in the independence of older adults. In this study we examine differences in the skeletal muscle transcriptome between healthy young and older subjects and (pre-)frail older adults. Additionally, we examine the effect of resistance-type exercise training on the muscle transcriptome in healthy older subjects and (pre-)frail older adults. Methods: Baseline transcriptome profiles were measured in muscle biopsies collected from 53 young, 73 healthy older subjects and 61 frail older subjects. Follow-up samples from these frail older subjects (31 samples) and healthy older subjects (41 samples) were collected after 6 months of progressive resistance-type exercise training. Frail older subjects trained twice per week and the healthy older subjects trained three times per week. Results: At baseline genes related to mitochondrial function and energy metabolism were differentially expressed between older and young subjects, as well as between healthy and frail older subjects. 307 genes were differentially expressed after training in both groups. Training affected expression levels of genes related to extracellular matrix, glucose metabolism and vascularization. Expression of genes that were modulated by exercise training were indicative of muscle strength at baseline. Genes that strongly correlated with strength belonged to the protocadherin gamma gene cluster (r=-0.73). Conclusions: Our data suggest significant remaining plasticity of ageing skeletal muscle to adapt to resistance-type exercise training. Some age-related changes in skeletal muscle gene expression appear to be partially reversed by prolonged resistance-type exercise training. The protocadherin gamma gene cluster may be related to muscle denervation and re-innervation in ageing muscle.

Item Type:	Article
Additional Information:	© 2016 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of the Society of Sarcopenia, Cachexia and Wasting Disorders This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Uncontrolled Keywords:	ageing,frailty,skeletal muscle,transcriptomics
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User:	Pure Connector
Date Deposited:	18 Feb 2016 12:00
Last Modified:	22 Oct 2022 00:31
URI:	https://ueaeprints.uea.ac.uk/id/eprint/57168
DOI:	10.1002/jcsm.12099

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