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Spalton, David J., Russell, Sarah L. 
ORCID: https://orcid.org/0000-0001-5615-3418, Evans-Gowing, Richard, Eldred, Julie A. and Wormstone, I. Michael ORCID: https://orcid.org/0000-0002-6423-7766
(2014)
Effect of total lens epithelial cell destruction on intraocular lens fixation in the human capsular bag.
Journal of Cataract and Refractive Surgery, 40 (2).
pp. 306-312.
ISSN 0886-3350
Abstract
PURPOSE: To evaluate the effect of complete destruction of lens epithelial cells (LECs) in the capsular bag on intraocular lens (IOL) stability. SETTING: School of Biological Sciences, University of East Anglia, Norwich, United Kingdom. DESIGN: Comparative evaluation. METHODS: An in vitro organ culture model using the bag zonule ciliary body complex isolated from fellow human donor eyes was prepared. A capsulorhexis and fiber extraction were performed, and an Acrysof IOL was implanted. Preparations were secured by pinning the ciliary body to a silicone ring and maintaining it in 6 mL Eagle minimum essential medium supplemented with 5% v/v fetal calf serum and 10 ng/mL transforming growth factor-beta 2 for 3 weeks or more. One bag of each pair was treated with 1 1,1,M thapsigargin to destroy all LECs. Observations of LEC growth were captured by phase-contrast microscopy, IOL stability by video microscopy, and endpoint analysis through scanning electron microscopy and immunocytochemistry. RESULTS The LECs in control capsular bags migrated centrally, closing the bag and fixating the IOL between the anterior and posterior capsules, as seen clinically. These events were not observed in the thapsigargin-treated group. After a period of controlled orbital movement, the IOL in the control group stabilized quicker than in the treated bags. There was no IOL rotation in the bag; however, the IOLs in the treated group rocked with axial movement. CONCLUSIONS: The LECs appeared to aid stabilization of current IOL designs in the capsular bag. The results have clinical implications for IOL design and for strategies to prevent posterior capsule opacification. (C) 2014 ASCRS and ESCRS
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | matrix metalloproteinases,equator rings,in-vitro,opacification,prevention,growth,implantation,rabbit,model,eyes |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science Faculty of Science > School of Natural Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Cells and Tissues |
| Depositing User: | Pure Connector |
| Date Deposited: | 22 Jan 2016 10:00 |
| Last Modified: | 27 Jan 2024 02:38 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/56745 |
| DOI: | 10.1016/j.jcrs.2013.06.030 |
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