MinION Analysis and Reference Consortium: Phase 1 data release and analysis

Ip, Camilla L. C., Loose, Matthew, Tyson, John R., de Cesare, Mariateresa, Brown, Bonnie L., Jain, Miten, Leggett, Richard M., Eccles, David A., Zalunin, Vadim, Urban, John M., Piazza, Paolo, Bowden, Rory J., Paten, Benedict, Mwaigwisya, Solomon, Batty, Elizabeth M., Simpson, Jared T., Snutch, Terrance P., Birney, Ewan, Buck, David, Goodwin, Sara, Jansen, Hans J., O'Grady, Justin and Olsen, Hugh E. (2015) MinION Analysis and Reference Consortium: Phase 1 data release and analysis. F1000Research, 4 (1075). ISSN 2046-1402

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Abstract

The advent of a miniaturized DNA sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools. The MinION™ Access Programme (MAP) was initiated by Oxford Nanopore Technologies™ in April 2014, giving public access to their USB-attached miniature sequencing device. The MinION Analysis and Reference Consortium (MARC) was formed by a subset of MAP participants, with the aim of evaluating and providing standard protocols and reference data to the community. Envisaged as a multi-phased project, this study provides the global community with the Phase 1 data from MARC, where the reproducibility of the performance of the MinION was evaluated at multiple sites. Five laboratories on two continents generated data using a control strain of Escherichia coli K-12, preparing and sequencing samples according to a revised ONT protocol. Here, we provide the details of the protocol used, along with a preliminary analysis of the characteristics of typical runs including the consistency, rate, volume and quality of data produced. Further analysis of the Phase 1 data presented here, and additional experiments in Phase 2 of E. coli from MARC are already underway to identify ways to improve and enhance MinION performance.

Item Type:	Article
Additional Information:	Date of Acceptance: 23/11/2015 © 2015 Ip CLC et al. This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Medical Microbiology (former - to 2018)
Related URLs:	http://f1000research.com/articles/4-1075...
Depositing User:	Pure Connector
Date Deposited:	02 Dec 2015 13:00
Last Modified:	19 Mar 2024 10:49
URI:	https://ueaeprints.uea.ac.uk/id/eprint/55633
DOI:	10.12688/f1000research.7201.1

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